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CABRI:ICLC Accession_number ICLC HTL04005 Cell_line_name A-498 Brief_description Species: human; Tissue: kidney; Tumor: adenocarcinoma Description Species: human female 52 years old; Tissue: kidney; Tumor: adenocarcinoma Depositor Dr. M. Viale, Pharmacology Dept, IST, Genoa, Italy. Bibliographic_reference - Morphology epithelial, grown as monolayer Culture_conditions continuous culture; MEM (EBSS) + 10% FBS + 2mM L-Glutamine + 1mM Na pyruvate; Split confluent cultures 1:3-1:6 three times a week using trypsin/EDTA (2-3 minutes); 37C, 5% CO2 Viruses Search for viruses was not performed Release_conditions Cell line available for distribution. For non-commercial investigative use only Hazard - Freezing_medium Culture medium + 50% FBS + 10% DMSO Sterility mycoplasma negative, HOECHST and PCR Further_bibliography J Natl Cancer Inst 1977;58:209-214 [PMID: 833871 ] J Natl Cancer Inst 1977;59:221-226 [PMID: 327080 ]

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